Influenza and SARS-Coronavirus Activating Proteases TMPRSS2 and HAT Are Expressed at Multiple Sites in Human Respiratory and Gastrointestinal Tracts
Identifieur interne : 001D02 ( Main/Exploration ); précédent : 001D01; suivant : 001D03Influenza and SARS-Coronavirus Activating Proteases TMPRSS2 and HAT Are Expressed at Multiple Sites in Human Respiratory and Gastrointestinal Tracts
Auteurs : Stephanie Bertram [Allemagne] ; Adeline Heurich [Allemagne] ; Hayley Lavender [Royaume-Uni] ; Stefanie Gierer [Allemagne] ; Simon Danisch [Allemagne] ; Paula Perin [Allemagne] ; Jared M. Lucas [États-Unis] ; Peter S. Nelson [États-Unis] ; Stefan Pöhlmann [Allemagne] ; Elizabeth J. Soilleux [Royaume-Uni]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Descripteurs français
- KwdFr :
- Acides sialiques (métabolisme), Activation enzymatique, Animaux, Appareil respiratoire (enzymologie), Appareil respiratoire (virologie), Expression des gènes, Grippe humaine (enzymologie), Grippe humaine (génétique), Grippe humaine (transmission), Grippe humaine (virologie), Humains, Lignée cellulaire, Oiseaux, Orthomyxoviridae (physiologie), Peptidyl-Dipeptidase A (génétique), Peptidyl-Dipeptidase A (métabolisme), Récepteurs viraux (génétique), Récepteurs viraux (métabolisme), Réservoirs d'agents pathogènes (médecine vétérinaire), Réservoirs d'agents pathogènes (virologie), Serine endopeptidases (génétique), Serine endopeptidases (métabolisme), Suidae, Syndrome respiratoire aigu sévère (enzymologie), Syndrome respiratoire aigu sévère (génétique), Syndrome respiratoire aigu sévère (transmission), Syndrome respiratoire aigu sévère (virologie), Tube digestif (enzymologie), Tube digestif (virologie), Virus du SRAS (physiologie).
- MESH :
- enzymologie : Appareil respiratoire, Grippe humaine, Syndrome respiratoire aigu sévère, Tube digestif.
- génétique : Grippe humaine, Peptidyl-Dipeptidase A, Récepteurs viraux, Serine endopeptidases, Syndrome respiratoire aigu sévère.
- médecine vétérinaire : Réservoirs d'agents pathogènes.
- métabolisme : Acides sialiques, Peptidyl-Dipeptidase A, Récepteurs viraux, Serine endopeptidases.
- physiologie : Orthomyxoviridae, Virus du SRAS.
- virologie : Appareil respiratoire, Grippe humaine, Réservoirs d'agents pathogènes, Syndrome respiratoire aigu sévère, Tube digestif.
- transmission : Activation enzymatique, Animaux, Expression des gènes, Grippe humaine, Humains, Lignée cellulaire, Oiseaux, Suidae, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Animals, Birds, Cell Line, Disease Reservoirs (veterinary), Disease Reservoirs (virology), Enzyme Activation, Gastrointestinal Tract (enzymology), Gastrointestinal Tract (virology), Gene Expression, Humans, Influenza, Human (enzymology), Influenza, Human (genetics), Influenza, Human (transmission), Influenza, Human (virology), Orthomyxoviridae (physiology), Peptidyl-Dipeptidase A (genetics), Peptidyl-Dipeptidase A (metabolism), Receptors, Virus (genetics), Receptors, Virus (metabolism), Respiratory System (enzymology), Respiratory System (virology), SARS Virus (physiology), Serine Endopeptidases (genetics), Serine Endopeptidases (metabolism), Severe Acute Respiratory Syndrome (enzymology), Severe Acute Respiratory Syndrome (genetics), Severe Acute Respiratory Syndrome (transmission), Severe Acute Respiratory Syndrome (virology), Sialic Acids (metabolism), Swine.
- MESH :
- chemical , genetics : Peptidyl-Dipeptidase A, Receptors, Virus, Serine Endopeptidases.
- enzymology : Gastrointestinal Tract, Influenza, Human, Respiratory System, Severe Acute Respiratory Syndrome.
- genetics : Influenza, Human, Severe Acute Respiratory Syndrome.
- chemical , metabolism : Peptidyl-Dipeptidase A, Receptors, Virus, Serine Endopeptidases, Sialic Acids.
- physiology : Orthomyxoviridae, SARS Virus.
- transmission : Influenza, Human, Severe Acute Respiratory Syndrome.
- veterinary : Disease Reservoirs.
- virology : Disease Reservoirs, Gastrointestinal Tract, Influenza, Human, Respiratory System, Severe Acute Respiratory Syndrome.
- Animals, Birds, Cell Line, Enzyme Activation, Gene Expression, Humans, Swine.
Abstract
The type II transmembrane serine proteases TMPRSS2 and HAT activate influenza viruses and the SARS-coronavirus (TMPRSS2) in cell culture and may play an important role in viral spread and pathogenesis in the infected host. However, it is at present largely unclear to what extent these proteases are expressed in viral target cells in human tissues. Here, we show that both HAT and TMPRSS2 are coexpressed with 2,6-linked sialic acids, the major receptor determinant of human influenza viruses, throughout the human respiratory tract. Similarly, coexpression of ACE2, the SARS-coronavirus receptor, and TMPRSS2 was frequently found in the upper and lower aerodigestive tract, with the exception of the vocal folds, epiglottis and trachea. Finally, activation of influenza virus was conserved between human, avian and porcine TMPRSS2, suggesting that this protease might activate influenza virus in reservoir-, intermediate- and human hosts. In sum, our results show that TMPRSS2 and HAT are expressed by important influenza and SARS-coronavirus target cells and could thus support viral spread in the human host.
Url:
DOI: 10.1371/journal.pone.0035876
PubMed: 22558251
PubMed Central: 3340400
Affiliations:
- Allemagne, Royaume-Uni, États-Unis
- Angleterre, Basse-Saxe, Oxfordshire, Washington (État)
- Göttingen, Hanovre, Oxford
- Université d'Oxford
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Le document en format XML
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xml:lang="en"><term>Gastrointestinal Tract</term><term>Influenza, Human</term><term>Respiratory System</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza, Human</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Grippe humaine</term><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term><term>Serine endopeptidases</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term><term>Serine Endopeptidases</term><term>Sialic Acids</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Réservoirs d'agents pathogènes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides sialiques</term><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term><term>Serine endopeptidases</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Orthomyxoviridae</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Orthomyxoviridae</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Influenza, Human</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Disease Reservoirs</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Appareil respiratoire</term><term>Grippe humaine</term><term>Réservoirs d'agents pathogènes</term><term>Syndrome respiratoire aigu sévère</term><term>Tube digestif</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Disease Reservoirs</term><term>Gastrointestinal Tract</term><term>Influenza, Human</term><term>Respiratory System</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Birds</term><term>Cell Line</term><term>Enzyme Activation</term><term>Gene Expression</term><term>Humans</term><term>Swine</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="fr"><term>Activation enzymatique</term><term>Animaux</term><term>Expression des gènes</term><term>Grippe humaine</term><term>Humains</term><term>Lignée cellulaire</term><term>Oiseaux</term><term>Suidae</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The type II transmembrane serine proteases TMPRSS2 and HAT activate influenza viruses and the SARS-coronavirus (TMPRSS2) in cell culture and may play an important role in viral spread and pathogenesis in the infected host. However, it is at present largely unclear to what extent these proteases are expressed in viral target cells in human tissues. Here, we show that both HAT and TMPRSS2 are coexpressed with 2,6-linked sialic acids, the major receptor determinant of human influenza viruses, throughout the human respiratory tract. Similarly, coexpression of ACE2, the SARS-coronavirus receptor, and TMPRSS2 was frequently found in the upper and lower aerodigestive tract, with the exception of the vocal folds, epiglottis and trachea. Finally, activation of influenza virus was conserved between human, avian and porcine TMPRSS2, suggesting that this protease might activate influenza virus in reservoir-, intermediate- and human hosts. In sum, our results show that TMPRSS2 and HAT are expressed by important influenza and SARS-coronavirus target cells and could thus support viral spread in the human host.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Neumann, G" uniqKey="Neumann G">G Neumann</name></author><author><name sortKey="Noda, T" uniqKey="Noda T">T Noda</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Skowronski, Dm" uniqKey="Skowronski D">DM Skowronski</name></author><author><name sortKey="Astell, C" uniqKey="Astell C">C Astell</name></author><author><name sortKey="Brunham, Rc" uniqKey="Brunham R">RC Brunham</name></author><author><name sortKey="Low, De" uniqKey="Low D">DE Low</name></author><author><name sortKey="Petric, M" uniqKey="Petric M">M Petric</name></author></analytic></biblStruct><biblStruct><analytic><author><name 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Bulthuis</name></author><author><name sortKey="Lely, At" uniqKey="Lely A">AT Lely</name></author><author><name sortKey="Navis, Gj" uniqKey="Navis G">GJ Navis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mossel, Ec" uniqKey="Mossel E">EC Mossel</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author><author><name sortKey="Jeffers, S" uniqKey="Jeffers S">S Jeffers</name></author><author><name sortKey="Edeen, Ke" uniqKey="Edeen K">KE Edeen</name></author><author><name sortKey="Wang, S" uniqKey="Wang S">S Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="To, Kf" uniqKey="To K">KF To</name></author><author><name sortKey="Lo, Aw" uniqKey="Lo A">AW Lo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gu, J" uniqKey="Gu J">J Gu</name></author><author><name sortKey="Gong, E" uniqKey="Gong E">E Gong</name></author><author><name sortKey="Zhang, B" uniqKey="Zhang B">B Zhang</name></author><author><name sortKey="Zheng, J" uniqKey="Zheng J">J Zheng</name></author><author><name sortKey="Gao, Z" uniqKey="Gao Z">Z Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, Ts" uniqKey="Kim T">TS Kim</name></author><author><name sortKey="Heinlein, C" uniqKey="Heinlein C">C Heinlein</name></author><author><name sortKey="Hackman, Rc" uniqKey="Hackman R">RC Hackman</name></author><author><name sortKey="Nelson, Ps" uniqKey="Nelson P">PS Nelson</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Basse-Saxe</li><li>Oxfordshire</li><li>Washington (État)</li></region><settlement><li>Göttingen</li><li>Hanovre</li><li>Oxford</li></settlement><orgName><li>Université d'Oxford</li></orgName></list><tree><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Bertram, Stephanie" sort="Bertram, Stephanie" uniqKey="Bertram S" first="Stephanie" last="Bertram">Stephanie Bertram</name></region><name sortKey="Danisch, Simon" sort="Danisch, Simon" uniqKey="Danisch S" first="Simon" last="Danisch">Simon Danisch</name><name sortKey="Gierer, Stefanie" sort="Gierer, Stefanie" uniqKey="Gierer S" first="Stefanie" last="Gierer">Stefanie Gierer</name><name sortKey="Heurich, Adeline" sort="Heurich, Adeline" uniqKey="Heurich A" first="Adeline" last="Heurich">Adeline Heurich</name><name sortKey="Perin, Paula" sort="Perin, Paula" uniqKey="Perin P" first="Paula" last="Perin">Paula Perin</name><name sortKey="Pohlmann, Stefan" sort="Pohlmann, Stefan" uniqKey="Pohlmann S" first="Stefan" last="Pöhlmann">Stefan Pöhlmann</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Lavender, Hayley" sort="Lavender, Hayley" uniqKey="Lavender H" first="Hayley" last="Lavender">Hayley Lavender</name></region><name sortKey="Soilleux, Elizabeth J" sort="Soilleux, Elizabeth J" uniqKey="Soilleux E" first="Elizabeth J." last="Soilleux">Elizabeth J. Soilleux</name></country><country name="États-Unis"><region name="Washington (État)"><name sortKey="Lucas, Jared M" sort="Lucas, Jared M" uniqKey="Lucas J" first="Jared M." last="Lucas">Jared M. Lucas</name></region><name sortKey="Nelson, Peter S" sort="Nelson, Peter S" uniqKey="Nelson P" first="Peter S." last="Nelson">Peter S. Nelson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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